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amylose resin beads  (New England Biolabs)


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    Structured Review

    New England Biolabs amylose resin beads
    Amylose Resin Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amylose+resin+beads/Amylose+Resin+High+Flow/pmc13084070-264-7-10
    Average 95 stars, based on 354 article reviews
    amylose resin beads - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: MAP4K1 and MAP4K2 regulate ABA-induced and Ca 2+ -mediated stomatal closure in Arabidopsis .
    Article Snippet: .. Amylose resin beads (New England Biolabs, MA) were added to the mixture and incubated for 3 hours at 4°C. ..

    Article Title: Tubby-like protein TaTLP5 enhances stripe rust resistance in wheat by regulating TaCAT1 protein degradation.
    Article Snippet: .. In the case of MBP-tagged proteins, the lysate was incubated with amylose resin beads (cat no.: E8037; New England Biolabs, Ipswich, MA, USA). ..

    Article Title: MAP4K1 and MAP4K2 regulate ABA-induced and Ca 2+ -mediated stomatal closure in Arabidopsis
    Article Snippet: .. Amylose resin beads (New England Biolabs, MA) were added to the mixture and incubated for 3 hours at 4°C. ..

    Article Title: Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicus
    Article Snippet: .. The supernatant was incubated with 20 μL amylose resin beads (New England Biolabs, E8022S) at 4°C for 3 h. Beads were washed at least five times with column buffer. .. Immunoblotting was performed using anti-Myc (BioLegend, 626808) and anti-NopM antibodies; anti-NopM antibodies were provided by Dr. Dawei Xin (Northeast Agriculture University, Harbin).

    Recombinant:

    Article Title: Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicus
    Article Snippet: .. Recombinant protein expression was induced with 0.5 mM IPTG, and cultures were grown at 18°C for 20 h. For phosphatase (λ-PPase) treatment, bacterial proteins were extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1 mM PMSF) and purified using amylose resin beads (New England Biolabs, E8022S). .. Beads were washed in washing buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40) and incubated in the absence or presence of 0.2 U/mL λ-PPase (New England Biolabs, P0753S) at 37°C for 10 min. Lysates were analyzed by immunoblotting using anti-Myc (BioLegend, 626808) and anti-FLAG (Sigma, F1804) antibodies.

    Expressing:

    Article Title: Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicus
    Article Snippet: .. Recombinant protein expression was induced with 0.5 mM IPTG, and cultures were grown at 18°C for 20 h. For phosphatase (λ-PPase) treatment, bacterial proteins were extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1 mM PMSF) and purified using amylose resin beads (New England Biolabs, E8022S). .. Beads were washed in washing buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40) and incubated in the absence or presence of 0.2 U/mL λ-PPase (New England Biolabs, P0753S) at 37°C for 10 min. Lysates were analyzed by immunoblotting using anti-Myc (BioLegend, 626808) and anti-FLAG (Sigma, F1804) antibodies.

    Lysis:

    Article Title: Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicus
    Article Snippet: .. Recombinant protein expression was induced with 0.5 mM IPTG, and cultures were grown at 18°C for 20 h. For phosphatase (λ-PPase) treatment, bacterial proteins were extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1 mM PMSF) and purified using amylose resin beads (New England Biolabs, E8022S). .. Beads were washed in washing buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40) and incubated in the absence or presence of 0.2 U/mL λ-PPase (New England Biolabs, P0753S) at 37°C for 10 min. Lysates were analyzed by immunoblotting using anti-Myc (BioLegend, 626808) and anti-FLAG (Sigma, F1804) antibodies.

    Purification:

    Article Title: Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicus
    Article Snippet: .. Recombinant protein expression was induced with 0.5 mM IPTG, and cultures were grown at 18°C for 20 h. For phosphatase (λ-PPase) treatment, bacterial proteins were extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1 mM PMSF) and purified using amylose resin beads (New England Biolabs, E8022S). .. Beads were washed in washing buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40) and incubated in the absence or presence of 0.2 U/mL λ-PPase (New England Biolabs, P0753S) at 37°C for 10 min. Lysates were analyzed by immunoblotting using anti-Myc (BioLegend, 626808) and anti-FLAG (Sigma, F1804) antibodies.

    Article Title: Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy.
    Article Snippet: GST-bound proteins were purified by lysing the E. coli cells with GST-lysis buffer (50 mM Tris–HCl (pH 8; Sigma-Aldrich, T1378-1KG), 250 mM NaCl (Sigma-Aldrich, 71 376), 1 mM EDTA (Roche, 10 708 984 001, Basel, Switzerland), 19 complete EDTA-free protease inhibitor cocktail (Roche, 5 056 489 001)) and isolating the proteins with Glutathione Sepharose 4B beads (Sigma Aldrich, GE17-075601; Uppsala, Sweden). .. MBP-tagged proteins were purified by lysing the E. coli cells in MBP-lysis buffer (20 mM Tris–HCl pH 7.4, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 19 complete EDTA-free protease inhibitor cocktail) and isolating the proteins with amylose-resin beads (NEB, E8021L; Ipswich, MA, USA), followed by elution with 10 mM maltose on Poly-Prep Chromatography Columns (Biorad, 7 311 550; Richmond, CA, USA) at 4 °C. .. For in vitro interaction assays, equal concentrations of GST- and MBP-tagged proteins were incubated together for 2 h at 4 °C and washed six times with GSTbuffer before heat inactivation and denaturation at 95 °C in Laemmli Sample Buffer (Bio-Rad, 1 610 747; Hercules, CA, USA) supplemented with DTT (Roche, 10 708 984 001) followed by immunoblotting.

    Article Title: TaGW2-TaVOZ1 module regulates wheat salt tolerance via both E3 ligase–dependent and –independent pathways
    Article Snippet: .. The proteins were purified using amylose resin beads (New England Biolabs) and Ni–nitrilotriacetic acid resin beads (Thermo Fisher Scientific), respectively. ..

    Article Title: TaFAR5-TaFAR3 module regulates cuticular wax biosynthesis and drought tolerance in wheat.
    Article Snippet: Plant cuticular waxes function as a protective barrier to mitigate environmental stresses, especially water deficit, although the molecular mechanisms and natural genetic variations underlying wax accumulation in crops remain unclear.. Our genome-wide association study (GWAS) of the contents of cuticular wax components in wheat seedlings demonstrated that allelic variations in TaFAR5, encoding a fatty acyl-CoA reductase, contribute to the differences in leaf cuticular wax accumulation.. Molecular and transgenic analyses revealed that variations in the TaFAR5 promoter affect the binding affinity between cis-regulatory elements and several transcription factors, including TaLBD16, TaERF12, TaNAC2, TaWRKY2, TaMYBC1, and TaNAC6, consequently modulating TaFAR5 expression.

    Protease Inhibitor:

    Article Title: Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy.
    Article Snippet: GST-bound proteins were purified by lysing the E. coli cells with GST-lysis buffer (50 mM Tris–HCl (pH 8; Sigma-Aldrich, T1378-1KG), 250 mM NaCl (Sigma-Aldrich, 71 376), 1 mM EDTA (Roche, 10 708 984 001, Basel, Switzerland), 19 complete EDTA-free protease inhibitor cocktail (Roche, 5 056 489 001)) and isolating the proteins with Glutathione Sepharose 4B beads (Sigma Aldrich, GE17-075601; Uppsala, Sweden). .. MBP-tagged proteins were purified by lysing the E. coli cells in MBP-lysis buffer (20 mM Tris–HCl pH 7.4, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 19 complete EDTA-free protease inhibitor cocktail) and isolating the proteins with amylose-resin beads (NEB, E8021L; Ipswich, MA, USA), followed by elution with 10 mM maltose on Poly-Prep Chromatography Columns (Biorad, 7 311 550; Richmond, CA, USA) at 4 °C. .. For in vitro interaction assays, equal concentrations of GST- and MBP-tagged proteins were incubated together for 2 h at 4 °C and washed six times with GSTbuffer before heat inactivation and denaturation at 95 °C in Laemmli Sample Buffer (Bio-Rad, 1 610 747; Hercules, CA, USA) supplemented with DTT (Roche, 10 708 984 001) followed by immunoblotting.

    Chromatography:

    Article Title: Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy.
    Article Snippet: GST-bound proteins were purified by lysing the E. coli cells with GST-lysis buffer (50 mM Tris–HCl (pH 8; Sigma-Aldrich, T1378-1KG), 250 mM NaCl (Sigma-Aldrich, 71 376), 1 mM EDTA (Roche, 10 708 984 001, Basel, Switzerland), 19 complete EDTA-free protease inhibitor cocktail (Roche, 5 056 489 001)) and isolating the proteins with Glutathione Sepharose 4B beads (Sigma Aldrich, GE17-075601; Uppsala, Sweden). .. MBP-tagged proteins were purified by lysing the E. coli cells in MBP-lysis buffer (20 mM Tris–HCl pH 7.4, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 19 complete EDTA-free protease inhibitor cocktail) and isolating the proteins with amylose-resin beads (NEB, E8021L; Ipswich, MA, USA), followed by elution with 10 mM maltose on Poly-Prep Chromatography Columns (Biorad, 7 311 550; Richmond, CA, USA) at 4 °C. .. For in vitro interaction assays, equal concentrations of GST- and MBP-tagged proteins were incubated together for 2 h at 4 °C and washed six times with GSTbuffer before heat inactivation and denaturation at 95 °C in Laemmli Sample Buffer (Bio-Rad, 1 610 747; Hercules, CA, USA) supplemented with DTT (Roche, 10 708 984 001) followed by immunoblotting.



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